HNF4A antibody (AA 280-329)

Catalog No. ABIN1532895

This Rabbit Polyclonal antibody specifically detects HNF4A in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for HNF4A antibody (AA 280-329) (ABIN1532895)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-HNF4A Antibody

Binding Specificity: AA 280-329

Purpose: HNF-4α Antibody

Specificity: HNF-4α Polyclonal Antibody detects endogenous levels of HNF-4α protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HNF4 alpha. AA range:280-329

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4 alpha

Background:

Synonyms: HNF4A, HNF4, NR2A1, TCF14, Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4

Background: The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012],

Gene Name: HNF4A

Protein Name: Hepatocyte nuclear factor 4-alpha

Molecular Weight: 52 kDa

Gene ID: 3172

OMIM: 125850

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process