SKP2 antibody (AA 366-415)

Catalog No. ABIN1533399

This Rabbit Polyclonal antibody specifically detects SKP2 in WB, IF, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SKP2 antibody (AA 366-415) (ABIN1533399)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Product Details anti-SKP2 Antibody

Binding Specificity: AA 366-415

Purpose: Skp2 p45 Antibody

Specificity: Skp2 p45 Polyclonal Antibody detects endogenous levels of Skp2 p45 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human SKP2/p45. AA range:366-415

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name: SKP2/p45

Background:

Synonyms: SKP2, FBXL1, S-phase kinase-associated protein 2, Cyclin-A/CDK2-associated protein p45, F-box protein Skp2, F-box/LRR-repeat protein 1, p45skp2

Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs.The protein encoded by this gene belongs to the Fbls class, in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and int

Gene Name: SKP2

Protein Name: S-phase kinase-associated protein 2

Molecular Weight: 47 kDa

Gene ID: 6502

OMIM: 601436

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases