HMGB1 antibody (AA 131-180)

Catalog No. ABIN1533598

The Rabbit Polyclonal anti-HMGB1 antibody has been validated for WB and ELISA. It is suitable to detect HMGB1 in samples from Human, Mouse and Rat.

Quick Overview for HMGB1 antibody (AA 131-180) (ABIN1533598)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HMGB1 Antibody

Binding Specificity: AA 131-180

Specificity:

HMGB1 Antibody detects endogenous levels of total HMGB1 protein.

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HMGB1.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:20000

Comment:

Unigene-Number: Hs.593339, Hs.644368, Hs.714718, (NCBI Gene Symbol: HMGB1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background:

Synonyms: High mobility group protein B1, High mobility group protein 1, HMG-1, HMG1

NCBI Gene Symbol: HMGB1

Molecular Weight: 24 kDa

Gene ID: 3146

OMIM: 163905

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome