PFKL antibody (AA 691-740)

Catalog No. ABIN1533639

This Rabbit Polyclonal antibody specifically detects PFKL in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PFKL antibody (AA 691-740) (ABIN1533639)

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PFKL Antibody

Binding Specificity: AA 691-740

Purpose: PFK-B Antibody

Specificity: PFK-B Polyclonal Antibody detects endogenous levels of PFK-B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human K6PL. AA range:691-740

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PFKL

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Background:

Synonyms: PFKL, 6-phosphofructokinase, liver type, Phosphofructo-1-kinase isozyme B, PFK-B, Phosphofructokinase 1, Phosphohexokinase

Background: This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014],

Gene Name: PFKL

Protein Name: 6-phosphofructokinase liver type

Molecular Weight: 85 kDa

Gene ID: 5211

OMIM: 171860

UniProt: P17858

Pathways: Negative Regulation of Hormone Secretion, Warburg Effect