Calreticulin antibody (AA 21-70)

Catalog No. ABIN1533682

The Rabbit Polyclonal anti-Calreticulin antibody is suitable to detect Calreticulin in samples from Human, Rat, Mouse and Monkey. It has been validated for WB, IHC, ELISA, FACS and IF.

Quick Overview for Calreticulin antibody (AA 21-70) (ABIN1533682)

Target: Calreticulin (CALR)

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Calreticulin Antibody

Binding Specificity: AA 21-70

Purpose: Calregulin Antibody

Specificity: Calregulin Polyclonal Antibody detects endogenous levels of Calregulin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CALR. AA range:21-70

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,Flow Cyt 1:50-200,IHC 1:100-500,IF ICC 1:100-500,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR

Background:

Synonyms: CALR, CRTC, Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60

Background: Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its

Gene Name: CALR

Protein Name: Calreticulin

Molecular Weight: 48 kDa

Gene ID: 811

OMIM: 109091

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response