MELK antibody (AA 431-480)

Catalog No. ABIN1533892

The Rabbit Polyclonal anti-MELK antibody is suitable to detect MELK in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for MELK antibody (AA 431-480) (ABIN1533892)

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MELK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MELK Antibody

Binding Specificity: AA 431-480

Purpose: MELK Antibody

Specificity: MELK Polyclonal Antibody detects endogenous levels of MELK protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MELK. AA range:431-480

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MELK

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Alternative Name: MELK

Background:

Synonyms: MELK, KIAA0175, Maternal embryonic leucine zipper kinase, hMELK, Protein kinase Eg3, pEg3 kinase, Protein kinase PK38, hPK38, Tyrosine-protein kinase MELK

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.,PTM:Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.,similarity:Contains 1 KA1 (kinase-associated) domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with ZNF622 and PPP1R8.,tissue specificity:Expressed in placenta, kidney, thymus, testis, ovary and intestine.,

Gene Name: MELK

Protein Name: Maternal embryonic leucine zipper kinase

Molecular Weight: 74 kDa

Gene ID: 9833

OMIM: 607025

UniProt: Q14680