MAP3K3 antibody (AA 101-150)

Catalog No. ABIN1534084

The Rabbit Polyclonal anti-MAP3K3 antibody is suitable to detect MAP3K3 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for MAP3K3 antibody (AA 101-150) (ABIN1534084)

Target: MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MAP3K3 Antibody

Binding Specificity: AA 101-150

Purpose: MEK Kinase-3 Antibody

Specificity: MEK Kinase-3 Polyclonal Antibody detects endogenous levels of MEK Kinase-3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MAP3K3. AA range:101-150

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MAP3K3

Target: MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))

Alternative Name: MAP3K3

Background:

Synonyms: MAP3K3, MAPKKK3, MEKK3, Mitogen-activated protein kinase kinase kinase 3, MAPK/ERK kinase kinase 3, MEK kinase 3, MEKK 3

Background: This gene product is a 626-amino acid polypeptide that is 96.5 % identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively, it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

Gene Name: MAP3K3

Protein Name: Mitogen-activated protein kinase kinase kinase 3

Molecular Weight: 70 kDa

Gene ID: 4215

OMIM: 602539

UniProt: Q99759

Pathways: MAPK Signaling