14-3-3 zeta antibody (AA 51-100)

Catalog No. ABIN1534237

The Rabbit Polyclonal anti-14-3-3 zeta antibody is suitable to detect 14-3-3 zeta in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC (p) and IF (p).

Quick Overview for 14-3-3 zeta antibody (AA 51-100) (ABIN1534237)

Target: 14-3-3 zeta (YWHAZ)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-14-3-3 zeta Antibody

Binding Specificity: AA 51-100

Purpose: 14-3-3 ζ Antibody

Specificity: 14-3-3 ζ Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta. AA range:51-100

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: 14-3-3 zeta

Background:

Synonyms: YWHAZ, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1

Background: histone cluster 1 H3 family member a(HIST1H3A) Homo sapiens Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Gene Name: YWHAZ

Protein Name: 14-3-3 protein zeta/delta

Molecular Weight: 27 kDa

OMIM: 601288

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location