ADD3 antibody (AA 431-480)

Catalog No. ABIN1534259

The Rabbit Polyclonal anti-ADD3 antibody (ABIN1534259) specifically detects ADD3 in WB, ELISA, IF and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ADD3 antibody (AA 431-480) (ABIN1534259)

Target: ADD3 (Adducin 3 (Gamma) (ADD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-ADD3 Antibody

Binding Specificity: AA 431-480

Purpose: Adducin γ Antibody

Specificity: Adducin γ Polyclonal Antibody detects endogenous levels of Adducin γ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ADD3. AA range:431-480

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ADD3

Target: ADD3 (Adducin 3 (Gamma) (ADD3))

Alternative Name: ADD3

Background:

Synonyms: ADD3, ADDL, Gamma-adducin, Adducin-like protein 70

Background: adducin 3(ADD3) Homo sapiens Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region i

Gene Name: ADD3

Protein Name: Gamma-adducin

Molecular Weight: 79 kDa

Gene ID: 120

OMIM: 601568

UniProt: Q9UEY8