CA5B antibody (AA 241-290)

Catalog No. ABIN1534729

The Rabbit Polyclonal anti-CA5B antibody is suitable to detect CA5B in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for CA5B antibody (AA 241-290) (ABIN1534729)

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA5B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CA5B Antibody

Binding Specificity: AA 241-290

Purpose: CA VB Antibody

Specificity: CA VB Polyclonal Antibody detects endogenous levels of CA VB protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CA5B. AA range:241-290

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for CA5B

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Alternative Name: CA5B

Background:

Synonyms: CA5B, Carbonic anhydrase 5B, mitochondrial, Carbonate dehydratase VB, Carbonic anhydrase VB, CA-VB

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. [provided by RefSeq, Jul 2008],

Gene Name: CA5B

Protein Name: Carbonic anhydrase 5B mitochondrial

Molecular Weight: 36 kDa

Gene ID: 11238

OMIM: 300230

UniProt: Q9Y2D0