EIF5B antibody (AA 1051-1100)

Catalog No. ABIN1534902

This Rabbit Polyclonal antibody specifically detects EIF5B in WB, IF, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EIF5B antibody (AA 1051-1100) (ABIN1534902)

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Product Details anti-EIF5B Antibody

Binding Specificity: AA 1051-1100

Purpose: eIF5B Antibody

Specificity: EIF5B Polyclonal Antibody detects endogenous levels of eIF5B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human EIF5B. AA range:1051-1100

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for EIF5B

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Alternative Name: EIF5B

Background:

Synonyms: EIF5B, IF2, KIAA0741, Eukaryotic translation initiation factor 5B, eIF-5B, Translation initiation factor IF-2

Background: Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B.This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008],

Gene Name: EIF5B

Protein Name: Eukaryotic translation initiation factor 5B

Molecular Weight: 138 kDa

Gene ID: 9669

OMIM: 606086

UniProt: O60841