MYSM1 antibody (AA 520-569)

Catalog No. ABIN1535215

The Rabbit Polyclonal anti-MYSM1 antibody is suitable to detect MYSM1 in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IHC and IF.

Quick Overview for MYSM1 antibody (AA 520-569) (ABIN1535215)

Target: MYSM1 (Myb-Like, SWIRM and MPN Domains 1 (MYSM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYSM1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MYSM1 Antibody

Binding Specificity: AA 520-569

Purpose: MYSM1 Antibody

Specificity: MYSM1 Polyclonal Antibody detects endogenous levels of MYSM1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MYSM1. AA range:520-569

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MYSM1

Target: MYSM1 (Myb-Like, SWIRM and MPN Domains 1 (MYSM1))

Alternative Name: MYSM1

Background:

Synonyms: MYSM1, KIAA1915, Histone H2A deubiquitinase MYSM1, 2A-DUB, Myb-like, SWIRM and MPN domain-containing protein 1

Background: catalytic activity:Ubiquitin C-terminal thioester + H(2)O = ubiquitin + a thiol.,domain:Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA.,function:Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the peptidase M67A family. MYSM1 subfamily.,similarity:Contains 1 MPN (JAB/Mov34) domain.,similarity:Contains 1 SANT domain.,similarity:Contains 1 SWIRM domain.,subunit:Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Binds histones.,

Gene Name: MYSM1

Protein Name: Histone H2A deubiquitinase MYSM1

Molecular Weight: 95 kDa

Gene ID: 114803

UniProt: Q5VVJ2

Pathways: Chromatin Binding