Lamin A/C antibody (Cleaved-Asn231)

Catalog No. ABIN1536093

This Rabbit Polyclonal antibody specifically detects Lamin A/C in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Lamin A/C antibody (Cleaved-Asn231) (ABIN1536093)

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-Lamin A/C Antibody

Binding Specificity: AA 212-261, Cleaved-Asn231

Purpose: Cleaved-Lamin A (N231) Antibody

Specificity: Cleaved-Lamin A (N231) Polyclonal Antibody detects endogenous levels of fragment of activated Lamin A protein resulting from cleavage adjacent to N231.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Lamin A. AA range:212-261

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin A

Background:

Synonyms: LMNA, LMN1, Prelamin-A/C

Background: lamin A/C(LMNA) Homo sapiens The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012],

Gene Name: LMNA

Protein Name: Prelamin-A/C

Molecular Weight: 50 kDa

Gene ID: 4000

OMIM: 115200

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus