Caspase 3 antibody (Cleaved-Asp175)

Catalog No. ABIN1536103

The Rabbit Polyclonal anti-Caspase 3 antibody is suitable to detect Caspase 3 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Caspase 3 antibody (Cleaved-Asp175) (ABIN1536103)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 126-175, Cleaved-Asp175

Purpose: Cleaved-Caspase-3 p17 (D175) Antibody

Specificity: Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-3 p17 protein resulting from cleavage adjacent to D175.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:126-175

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase 3

Background:

Synonyms: CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Background: SMAD family member 3(SMAD3) Homo sapiens The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],

Gene Name: CASP3

Protein Name: Caspase3

Molecular Weight: 17 kDa

OMIM: 600636

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation