F12 antibody (Cleaved-Arg372)

Catalog No. ABIN1536117

This Rabbit Polyclonal antibody specifically detects F12 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for F12 antibody (Cleaved-Arg372) (ABIN1536117)

Target: F12 (Coagulation Factor XII (F12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This F12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-F12 Antibody

Binding Specificity: AA 323-372, Cleaved-Arg372

Purpose: Cleaved-Factor XII HC (R372) Antibody

Specificity: Cleaved-Factor XII HC (R372) Polyclonal Antibody detects endogenous levels of fragment of activated Factor XII HC protein resulting from cleavage adjacent to R372.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FA12. AA range:323-372

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for F12

Target: F12 (Coagulation Factor XII (F12))

Background:

Synonyms: F12, Coagulation factor XII, Hageman factor, HAF

Background: This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activat

Gene Name: F12

Protein Name: Coagulation factor XII

Molecular Weight: 41 kDa

Gene ID: 2161

OMIM: 610619

UniProt: P00748

Pathways: Complement System