Prostate Specific Antigen antibody

Catalog No. ABIN1543584

This anti-Prostate Specific Antigen antibody is a Mouse Monoclonal antibody detecting Prostate Specific Antigen in ELISA, WB, FACS and DB. Suitable for Human. Independently validated for use in Flow Cytometry.

Quick Overview for Prostate Specific Antigen antibody (ABIN1543584)

Target: Prostate Specific Antigen (PSA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prostate Specific Antigen antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB)

Clone: A5D5-1

Product Details anti-Prostate Specific Antigen Antibody

Purification: Protein A affinity column

Immunogen: Native PSA Antigen

Isotype: IgG1

Application Details

Comment:

Other Applications have not been tested.

Restrictions: For Research Use only

Independent Validation (FACS)

Validation #029617 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029617

Date: 02/22/2014

Lot Number: FAPS-A5D5-0003

Method validated: Flow Cytometry

Positive Control: LNCaP cells

Negative Control: - DU145 cells - Jurkat cells

Notes: Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.

Validation Images

Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).

Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).

Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).

Full Methods

Primary Antibody:

• Antibody: Prostate Specific Antigen (PSA)
• Catalog number: ABIN1543584
• Lot number: FAPS-A5D5-0003

Secondary Antibody:

• Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647

Full Protocol:

• Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
• Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
• An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
• Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
• Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
• 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
• Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 0.3 mL PBS/BSA
• Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Experimental Notes: None

Handling

Format: Liquid

Concentration: 1.8 mg/mL

Buffer: PBS, pH 7.4, 0.09% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: PSA

Pathways: Complement System