BRCA1 antibody

Catalog No. ABIN155101

This Rabbit Polyclonal antibody specifically detects BRCA1 in WB, IHC (p) and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for BRCA1 antibody (ABIN155101)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-BRCA1 Antibody

Specificity: This antibody detects BRCA1.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide surrounding amino acid 1852 of Human BRCA1

Application Details

Application Notes: Western Blot: 1-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.01 % Thimerosal, 0.5 % BSA

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1 / RNF53

Background: BRCA1 is a breast cancer susceptibility gene that was localized to chromosome 17q. Mutations within this gene are believed to account for approximately 45 % of families with high incidence of breast cancer and at least 80 % of families with increased incidence of both earlyonset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. Like many other genes involved in familial cancer, BRCA1 appears to encode a tumor suppressor, a protein that acts as a negative regulator of tumor growth.Synonyms: Breast cancer type 1 susceptibility protein, RING finger protein 53

Gene ID: 672

NCBI Accession: NP_009225

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus