HMOX1 antibody (N-Term)

Catalog No. ABIN155124

The Rabbit Polyclonal anti-HMOX1 antibody (ABIN155124) specifically detects HMOX1 in WB, IP and IHC (p). The antibody is reactive with Human, Rat, Mouse and Hamster samples.

Quick Overview for HMOX1 antibody (N-Term) (ABIN155124)

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Reactivity: Human, Rat, Mouse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMOX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HMOX1 Antibody

Binding Specificity: N-Term

Specificity: The antibody recognizes ~32 kDa Heme-Oxygenase-1.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide surrounding amino acid near the N-terminus of Human HO-1

Application Details

Application Notes: Western Blot: 0.5-2 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, 0.5 % BSA, 30 % Glycerol, 0.01 % Thimerosal

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for HMOX1

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Alternative Name: Heme Oxygenase 1 / HMOX1

Background: Heme oxygenase-1 (HO-1) or HSP32 is the inducible isoform of heme oxygenase which catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, iron and biliverdin that is immediately reduced to bilirubin. To date, three heme oxygenase isoforms HO-1, HO-2 and HO-3 have been identified. HO-1, also known as Hsp32, a major heat shock/stress response protein, is ubiquitous and its mRNA as well as its activity can be increased several-fold by heme, other metalloporphyrins, transition metals and stimuli that induce cellular stress. The 5'-untranslated region (UTR) of HO-1 has several consensus regulatory elements that include sites for activator protein 1 (AP-1), metal responsive element (MRE), oncogene c-myc/max heterodimer binding site (Myc/Max), antioxidant response element (ARE) and GC box binding (Sp1). HO-1 expression has been shown to increase in benign postatic hyperplasia (BPH) and malignant prostate tissue suggesting a role for this stress protein in the pathogenesis of BPH and prostate cancer.Synonyms: HO, HO-1, HO1

Gene ID: 3162

NCBI Accession: NP_002124

UniProt: P09601

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome