TIE1 antibody

Catalog No. ABIN155171

Product Details anti-TIE1 Antibody

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIE1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Specificity: This antibody will detect native TIE-1 in ELISA experiments and on the surface of different cell types.

Purification: Protein G chromatography

Immunogen: Recombinant human soluble TIE-1 protein

Clone: 6F12

Isotype: IgG1

Application Details

Application Notes: ELISA: 1-15 μg/mL. Western blot: 1-2 μg/mL. FACS. Cell sorting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS, pH 7.4 without preservatives or stabilizers

Preservative: Without preservative

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!

Target Details for TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: TIE1 (TIE1 Products)

Background: TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.Synonyms: TIE, Tie-1, Tyrosine-protein kinase receptor Tie-1

Gene ID: 7075

NCBI Accession: NP_005415

UniProt: P35590

Pathways: RTK Signaling