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14-3-3 zeta antibody (pSer58)

The Rabbit Polyclonal anti-14-3-3 zeta antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect 14-3-3 zeta in samples from Human, Mouse and Rat.
Catalog No. ABIN1574324

Quick Overview for 14-3-3 zeta antibody (pSer58) (ABIN1574324)

Target

See all 14-3-3 zeta (YWHAZ) Antibodies
14-3-3 zeta (YWHAZ)

Reactivity

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Human, Mouse, Rat

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This 14-3-3 zeta antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • Binding Specificity

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    pSer58

    Specificity

    Epitopes for Species: H:S58,M:S58,R:S58

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

    Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-SP-W-R).
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C / 1 year

    Expiry Date

    12 months
  • Target

    14-3-3 zeta (YWHAZ)

    Alternative Name

    14-3-3 zeta

    Background

    14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ

    UniProt

    P63104

    Pathways

    Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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