MAPT antibody

Catalog No. ABIN1580435

The Chicken Polyclonal anti-MAPT antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect MAPT in samples from Human.

Quick Overview for MAPT antibody (ABIN1580435)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MAPT Antibody

Purification: IgY preparation

Isotype: IgY

Application Details

Application Notes: Try at dilutions of 1:1,000 and higher for immunofluorescence. For western blots try at 1:10,000. A suitable control tissue is rat spinal cord or peripheral nerve homogenate.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: Microtubule Associated Protein tau (MAPT)

Background: Tau is a relatively low molecular weight member of the microtubule associated protein or MAP family. Most of these proteins were discovered since microtubules can be polymerized in cell homogenates and pelleted out by centrifugation, typically taking MAP proteins with them. This early work showed that tau protein facilitated the polymerization of microtubules, and was therefore given the name tau, the Greek letter tau, since it promoted tubule formation. The protein is now usually referred to simply as tau or by the HGNC name which is MAPT. Tau is heavily concentrated in axons of neurons, but may also be found in dendrites and in some non neuronal cells. Much interest has focused on tau as it is a major component of the neurofibrillary tangles of Alzheimer's disease. Tau in neurofibrillary tangles is typically heavily and aberrantly phosphorylated, and it is believed that phosphorylation may be involved in tangle formation. In addition, numerous different point mutations in the tau gene are causative of Fronto-temporal dementia with Parkinsonism linked to chromosome 17 (FTDP-17). There is one mammalian tau gene which produces at least 9 different proteins by alternate transcription. In the central nervous system 6 isoforms predominant which either include or do not include three short exon coded inserts. These proteins range is size from 352-441 amino acids and run on SDS-PAGE gels as multiple bands ranging from 48-67 kDa. In peripheral nervous system a form called big tau prodominates, another alternate transcript which includes a 254 amino acid insert. This form of tau is found in small amounts in the brain also, in cranial nerve motor nuclei and sensory processes of most sensory ganglia, and runs on SDS-PAGE with an apparent molecular weight of 100 kDa. Each tau protein contains 3 or 4 copies of an 18 amino acid peptide which are responsible for binding to the microtubules and are similar to those found in MAP2 and other members of the MAP family. Tau is a highly charged acidic protein with few hydrophobic residues which belongs to the family of intrinsically unstructured proteins. As with GAP43, MARCKS and several other similar proteins, tau isoforms run on SDS-PAGE much more slowly than expected from their actual molecular weight. Our antibody was made against a recombinant construct expressed in and purified from E. coli and which corresponded to the shortest version of the various tau proteins, so the antibody is expected to bind to all tau isoforms. The HGNC name for this protein is MAPT.

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size