TIE1 antibody (Biotin)

Catalog No. ABIN1589694

This anti-TIE1 antibody is a Mouse Monoclonal antibody detecting TIE1 in ELISA. Suitable for Human.

Quick Overview for TIE1 antibody (Biotin) (ABIN1589694)

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIE1 antibody is conjugated to Biotin

Application: ELISA

Clone: 6F12

Product Details anti-TIE1 Antibody (Biotin)

Purpose: TIE-1 antibody

Specificity: Recombinant human soluble extracellular TIE-1

Cross-Reactivity (Details): The unconjugated antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments and Western blotting.

Characteristics: Chromosomal location: 1p34-p33
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular TIE-1 as the immunizing antigen. The unconjugated antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments and Western blotting.

Purification: Mouse IgG1 antibody (#6F12) from hybridomas was purified from cell culture supernatant by Protein G chromatography and then biotinylated using a standard protocol.

Immunogen: Recombinant human soluble extracellular TIE-1

Isotype: IgG1

Application Details

Application Notes: ELISA: Use at 1-15 μg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: TIE-1

Background: TIE1, TIE, JTK14,Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

Gene ID: 7075

NCBI Accession: NM_005424, NP_005415

UniProt: P35590

Pathways: RTK Signaling