VEGFA antibody

Catalog No. ABIN1589946

The Rabbit Polyclonal anti-VEGFA antibody (ABIN1589946) specifically detects VEGFA in WB, ELISA and IHC (p). The antibody is reactive with Mouse samples.

Quick Overview for VEGFA antibody (ABIN1589946)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-VEGFA Antibody

Purpose: Rabbit Anti-Mouse VEGF-A Polyclonal Antibody

Specificity: Recombinant mouse VEGF164

Characteristics: Chromosomal location: 17C, 17 24.2cM
Produced from sera of rabbits immunized with highly pure recombinant murine VEGF164.

Purification: Anti-murine VEGF-A was purified by affinity chromatography with immobilized Protein A.

Immunogen: Recombinant mouse VEGF164 (ABIN1589664 and ABIN1589665)

Isotype: IgG

Application Details

Application Notes: Western Blot: use at 2-5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF-A

Background:

Synonyms: Vegfa, Vpf, Vegf, Vegf120, Vegf164, Vegf188

Description: Mouse VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors, fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant murine VEGF (isoform 164) is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 164 amino acid polypeptide chains.

Gene ID: 22339

NCBI Accession: NM_001025250, NP_001020421

UniProt: Q00731

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling