EEF1E1 antibody (Internal Region)

Catalog No. ABIN1589993

Product Details anti-EEF1E1 Antibody

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Binding Specificity: Internal Region

Reactivity: Human, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This EEF1E1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: EEF1E1 / AIMP3 / p18

Sequence: SKGNKYSAQG ERQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_004271.1, NP_001129122.1).

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 5 μg/mL.

Western Blot: Approx 19 kDa band observed in lysates of cell lines HepG2 and K562 (calculated MW of 19.8 kDa according to NP_004271.1) and in Pig Testis lysates (calculated MW of 19.8 kDa according to NP_001230474.1). Recommended concentration: 0.1-0.3 μg/mL

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EEF1E1

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Alternative Name: EEF1E1 (EEF1E1 Products)

Synonyms: p18 antibody, AIMP3 antibody, P18 antibody, 1110003A02Rik antibody, eukaryotic translation elongation factor 1 epsilon 1 antibody, eukaryotic translation elongation factor 1 epsilon 1 L homeolog antibody, EEF1E1 antibody, eef1e1.L antibody, eef1e1 antibody, Eef1e1 antibody

Background: EEF1E1, eukaryotic translation elongation factor 1 epsilon 1, AIMP3, P18, ARS-interacting multifunctional protein 3, aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, eukaryotic translation elongation factor 1 epsilon-1

Gene ID: 9521

NCBI Accession: NP_004271, NP_001129122

Pathways: Positive Regulation of Response to DNA Damage Stimulus