Patched 1 antibody (AA 266-280)

Catalog No. ABIN1590007

This Goat Polyclonal antibody specifically detects Patched 1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for Patched 1 antibody (AA 266-280) (ABIN1590007)

Target: Patched 1 (PTCH1)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Patched 1 Antibody

Binding Specificity: AA 266-280

Purpose: PTCH1 (aa266-280)

Sequence: EELKKINYQV DSWEE

Specificity: This antibody is expected to recognize all reported isoforms (NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077073.1). Reported variants represent identical protein: NP_001077075.1, NP_001077074.1, NP_001077073.1, NP_001077076.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EELKKINYQVDSWEE, from the C or N Terminus of the protein sequence according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077073.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 8 μg/mL.

Western Blot: Approx 140 kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159 kDa according to NP_032983.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: PTCH1

Background: PTCH1, patched 1, BCNS, FLJ26746, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched homolog 1, protein patched homolog 1

Gene ID: 5727, 19206, 89830

NCBI Accession: NP_000255, NP_001077072, NP_001077071, NP_001077073

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation