SPO11 antibody (Internal Region)

Catalog No. ABIN1590016

This Goat Polyclonal antibody specifically detects SPO11 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for SPO11 antibody (Internal Region) (ABIN1590016)

Target: SPO11 (SPO11 Meiotic Protein Covalently Bound To DSB Homolog (SPO11))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SPO11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SPO11 Antibody

Binding Specificity: Internal Region

Purpose: SPO11

Sequence: NTYATKRDIY YTDSQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_036576.1, NP_937998.1)

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38 kDa band observed in Human Testis lysates (calculated MW of 40.4 kDa according to NP_937998.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SPO11

Target: SPO11 (SPO11 Meiotic Protein Covalently Bound To DSB Homolog (SPO11))

Alternative Name: SPO11

Background: SPO11, SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae), MGC39953, SPO11 meiotic protein covalently bound to DSB-like, SPO11, meiotic protein covalently bound to DSB (S. cerevisiae)-like, meiotic recombination protein SPO11

Gene ID: 23626

NCBI Accession: NP_036576, NP_937998