E2F4 antibody (Internal Region)

Catalog No. ABIN1590023

The Goat Polyclonal anti-E2F4 antibody is suitable to detect E2F4 in samples from Human and Pig. It has been validated for WB and ELISA.

Quick Overview for E2F4 antibody (Internal Region) (ABIN1590023)

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Reactivity: Human, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This E2F4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-E2F4 Antibody

Binding Specificity: Internal Region

Purpose: Transcription factor E2F4

Sequence: PKELSEIFDP TR

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 44.0 kDa according to NP_001941.2). Recommended concentration: 0.1-0.3 μg/mL. Approx 40+50 kDa bands observed in Pig Brain and Heart lysates (calculated MW of

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Alternative Name: E2F4

Background: E2F4, E2F transcription factor 4, p107/p130-binding, E2F-4, p107/p130-binding protein, transcription factor E2F4

Gene ID: 1874, 104394, 100360427

NCBI Accession: NP_001941

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size