MCF2L antibody (Internal Region)

Catalog No. ABIN1590041

The Goat Polyclonal anti-MCF2L antibody is suitable to detect MCF2L in samples from Human. It has been validated for WB and ELISA.

Quick Overview for MCF2L antibody (Internal Region) (ABIN1590041)

Target: MCF2L (MCF.2 Cell Line Derived Transforming Sequence-Like (MCF2L))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MCF2L antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-MCF2L Antibody

Binding Specificity: Internal Region

Purpose: MCF2L

Sequence: QLDNQATVQR

Specificity: This antibody is expected to recognize both reported isoforms (NP_001106203.1, NP_079255.3).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in lysates of cell line Jurkat and of Human Thymus (calculated MW of 131 kDa according to NP_001106203.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MCF2L

Target: MCF2L (MCF.2 Cell Line Derived Transforming Sequence-Like (MCF2L))

Alternative Name: MCF2L

Background: MCF2L, MCF.2 cell line derived transforming sequence-like, ARHGEF14, DBS, FLJ12122, KIAA0362, OST, DBL's big sister, MCF2 transforming sequence-like protein, guanine nucleotide exchange factor DBS

Gene ID: 23263, 17207, 117020

NCBI Accession: NP_001106203, NP_079255

Pathways: Neurotrophin Signaling Pathway, Metabolism of Steroid Hormones and Vitamin D, Thyroid Hormone Synthesis