SOX10 antibody (Internal Region)

Catalog No. ABIN1590052

This Goat Polyclonal antibody specifically detects SOX10 in WB, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SOX10 antibody (Internal Region) (ABIN1590052)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SOX10 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Grade: Verified

Product Details anti-SOX10 Antibody

Binding Specificity: Internal Region

Purpose: SOX10

Sequence: DAKAQVKTET AGPQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Human Brain (Cerebellum) and approx. 52 kDa in Mouse Brain lysates (calculated MW of 49.9 kDa according to Human NP_008872.1 and Mouse NP_035567.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation 1

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus of KNRK cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Alternative Name: SOX10

Background: SOX10, SRY (sex determining region Y)-box 10 , DOM, MGC15649, WS4, OTTHUMP00000028515, SRY-related HMG-box gene 10, dominant megacolon, mouse, human homolog of

Gene ID: 6663

NCBI Accession: NP_008872

Pathways: Chromatin Binding