HIC1 antibody (AA 193-204)

Catalog No. ABIN1590055

This Goat Polyclonal antibody specifically detects HIC1 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for HIC1 antibody (AA 193-204) (ABIN1590055)

Target: HIC1 (Hypermethylated in Cancer 1 (HIC1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HIC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-HIC1 Antibody

Binding Specificity: AA 193-204

Purpose: HIC1 (aa193-204)

Sequence: NTHCAELYAS GP

Specificity: This antibody is expected to recognize both reported isoforms (NP_006488.2, NP_001091672.1).

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in Mouse Testis lysates (calculated MW of 76.8 kDa according to Mouse NP_001091673.1 ). Recommended concentration: 2-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HIC1

Target: HIC1 (Hypermethylated in Cancer 1 (HIC1))

Alternative Name: HIC1

Background: HIC1, hypermethylated in cancer 1, ZBTB29, ZNF901, hic-1, hypermethylated in cancer 1 protein, zinc finger and BTB domain-containing protein 29

Gene ID: 3090, 15248, 303310

NCBI Accession: NP_006488, NP_001091672

Pathways: Positive Regulation of Response to DNA Damage Stimulus