EDF1 antibody (Internal Region)

Catalog No. ABIN1590072

The Goat Polyclonal anti-EDF1 antibody (ABIN1590072) specifically detects EDF1 in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for EDF1 antibody (Internal Region) (ABIN1590072)

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EDF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-EDF1 Antibody

Binding Specificity: Internal Region

Purpose: EDF1

Sequence: NKQHSITKNT AKLDR

Specificity: This antibody is expected to recognize both reported isoforms (NP_003783.1, NP_694880.1).

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining of select lymphocytes in the marginal zone. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 17 kDa band observed in lysates of cell line A549 (calculated MW of 16.4 kDa according to NP_003783.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EDF1

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Alternative Name: EDF1

Background: EDF1, endothelial differentiation-related factor 1, EDF-1, MBF1, multiprotein bridging factor 1

Gene ID: 8721, 59022, 678956

NCBI Accession: NP_003783, NP_694880