E2F1 antibody (AA 314-327)

Catalog No. ABIN1590108

This anti-E2F1 antibody is a Goat Polyclonal antibody detecting E2F1 in WB and ELISA. Suitable for Human.

Quick Overview for E2F1 antibody (AA 314-327) (ABIN1590108)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-E2F1 Antibody

Binding Specificity: AA 314-327

Purpose: Transcription factor E2F1 (aa314-327)

Sequence: QEVTSEEENR ATDS

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 46.9 kDa according to NP_005216.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: E2F1, E2F transcription factor 1, E2F-1, RBAP1, RBBP3, RBP3, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, retinoblastoma-associated protein 1, retinoblastoma-binding protein 3, transcription factor E2F1

Gene ID: 1869

NCBI Accession: NP_005216

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy