PLK3 antibody (AA 320-333)

Catalog No. ABIN1590109

This Goat Polyclonal antibody specifically detects PLK3 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for PLK3 antibody (AA 320-333) (ABIN1590109)

Target: PLK3 (Polo-Like Kinase 3 (PLK3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PLK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PLK3 Antibody

Binding Specificity: AA 320-333

Purpose: polo-like kinase 3 (aa320-333)

Sequence: PDRLPISSCV TVPD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows vesicular staining in the cystoplasm of collective tubules. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 70 kDa band observed in lysates of cell line HeLa (calculated MW of 71.6 kDa according to NP_004064.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at rroom temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PLK3

Target: PLK3 (Polo-Like Kinase 3 (PLK3))

Alternative Name: PLK3

Background: PLK3, polo-like kinase 3, CNK, FNK, PRK, FGF-inducible kinase, PLK-3, cytokine-inducible kinase, cytokine-inducible serine/threonine-protein kinase, proliferation-related kinase, serine/threonine-protein kinase PLK3

Gene ID: 1263

NCBI Accession: NP_004064

Pathways: Regulation of long-term Neuronal Synaptic Plasticity