CPY antibody (Carboxypeptidase Y)

Details for Product anti-CPY Antibody No. ABIN96043
Antigen
Reactivity
Yeast (Saccharomyces cerevisiae)
15
Host
Rabbit
15
Clonality
Polyclonal
Conjugate
This CPY antibody is un-conjugated
4
2
Application
ELISA
14
12
9
5
4
3
3
3
3
1
Options
Immunogen Carboxypeptidase Y [Baker’s Yeast]
Immunogentype:Native
Characteristics Concentration Definition: by Refractometry
Antigen
Alternative Name Carboxypeptidase Y (CPY Antibody Abstract)
Background Synonyms: Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody
Gene ID 855343
UniProt P00729
Application Notes This product has been assayed against 1.0 µg of Carboxypeptidase Y [Baker’s Yeast] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. 
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 90 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Image no. 1 for anti-Carboxypeptidase Y (CPY) antibody (ABIN96043) Both the antiserum and IgG fractions of anti-Carboxypeptidase Y (Baker's Yeast) are s...
Product cited in: Martinez Benitez, Stolz, Becher, Wolf: "Mnl2, a novel component of the ER associated protein degradation pathway." in: Biochemical and biophysical research communications, 2011 (PubMed). Method employed by authors: Immunoprecipitation (IP) Further details: 900 ul of the supernatant was transferred to a microtube containing 3 ul of polyclonal CPY antibody for immunoprecipitation

Ohashi, Munro: "Membrane delivery to the yeast autophagosome from the Golgi-endosomal system." in: Molecular biology of the cell, Vol. 21, Issue 22, pp. 3998-4008, 2011 (PubMed).

von Numers, Survila, Aalto, Heino, Palva, Li: "Requirement of a Homolog of Glucosidase II {beta}-Subunit for EFR-Mediated Defense Signaling in Arabidopsis thaliana." in: Molecular plant, 2010 (PubMed).