Ascorbate Oxidase antibody
AO
Reactivity: Pumpkin
ELISA, WB, IP
Host: Rabbit
Polyclonal
unconjugated
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- Target See all Ascorbate Oxidase (AO) products
- Ascorbate Oxidase (AO)
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Reactivity
- Pumpkin
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Ascorbate Oxidase antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunoprecipitation (IP)
- Cross-Reactivity (Details)
- Cross reactivity against Ascorbate Oxidase from other sources is unknown.
- Purity
- Anti-ASCORBATE OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita].
- Endotoxin Level
- Low Endotoxin : No
- Immunogen
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Ascorbate Oxidase [cucurbita]
Immunogen Type: Native Protein - Isotype
- IgG
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- Application Notes
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ELISA Dilution: 1:25.000 - 1:125.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:2.000 - 1:10.000 - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Handling Advice
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- Expiry Date
- Expiration date is one (1) year from date of opening.
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- Target
- Ascorbate Oxidase (AO)
- Alternative Name
- Ascorbate Oxidase (AO Products)
- Synonyms
- AO antibody, ascorbate oxidase antibody, L-ascorbate oxidase antibody, AO antibody, LOC9310099 antibody
- Background
- Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3
- UniProt
- P24792
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