GLUD1 antibody (Biotin)

Catalog No. ABIN1607789

Product Details anti-GLUD1 Antibody (Biotin)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Glutamate Dehydrogenase from other sources may occur but have not been specifically determined.

Purity: Anti-Glutamate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver].

Endotoxin Level: Low Endotoxin : No

Immunogen: Glutamate Dehydrogenase [Bovine Liver]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Glutamate Dehydrogenase has been assayed against 1.0 µg of Glutamate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: Glutamate Dehydrogenase (GLUD1 Products)

Synonyms: GDH antibody, gdh1 antibody, GDH 1 antibody, glud1 antibody, GLUD1 antibody, cb719 antibody, wu:fb16e02 antibody, wu:fb58f12 antibody, wu:fe37f03 antibody, wu:fj43f02 antibody, zgc:192851 antibody, zgc:55630 antibody, GDH1 antibody, GLUD antibody, AI118167 antibody, Gdh-X antibody, Glud antibody, Gludl antibody, Ac2-281 antibody, Gdh1 antibody, Gludeha antibody, MRG-2 antibody, GLUTAMATE DECARBOXYLASE 1 antibody, GLUTAMATE DEHYDROGENASE 1 antibody, MRG7.13 antibody, MRG7_13 antibody, glutamate dehydrogenase 1 antibody, C2H1orf130 antibody, legdh1 antibody, cb622 antibody, wu:fc33g09 antibody, wu:fc66a10 antibody, zgc:77186 antibody, glutamate dehydrogenase 1 antibody, glutamate dehydrogenase 1 S homeolog antibody, glutamate dehydrogenase 1, mitochondrial antibody, glutamate dehydrogenase 1b antibody, glutamate dehydrogenase antibody, non-compact myelin associated protein antibody, glutamate dehydrogenase 1a antibody, GLUD1 antibody, GDH1 antibody, glud1 antibody, glud1.S antibody, LOC693461 antibody, glud1b antibody, Glud1 antibody, HACJB3_RS00320 antibody, LOC100587725 antibody, NCMAP antibody, gdh1 antibody, glud1a antibody

Background: Anti-glutamate dehydrogenase antibody recognizes the glutamate dehydrogenase protein. Glutamate dehydrogenase is an enzyme that is present in the mitochondria of eukaryotes. Glutamate dehydogenase plays a role in urea synthesis by converting glutamate to α-ketoglutarate, and vice versa. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals as glutamate dehydrogenase equilibrium favours the production of ammonia and α-ketoglutarate. Glutamate dehydrogenase also has a very high affinity for ammonia. Therefore toxic levels of ammonia would have to be present in the body for the reverse reaction to proceed. In bacteria, the ammonia is assimilated to amino acids via glutamate and amidotransferases. In plants, the enzyme can work in either direction depending on environment and stress. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections. Anti-glutamate dehydrogenase antibody is suitable for researchers in Cell Biology and Immunology. Synonyms: Glutamate dehydrogenase 1, mitochondrial GDH 1 EC=1.4.1.3

Gene ID: 281785, 32880221

UniProt: P00366

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect