Calnexin antibody (C-Term)

Catalog No. ABIN1607882

Supplier Product No.: 600-401-j15

Product Details anti-Calnexin Antibody

Target: Calnexin (CANX)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Chicken, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-j15

Supplier: Rockland

Purpose: Calnexin Antibody

Cross-Reactivity (Details): This antibody is specific towards calnexin.

Characteristics: Synonyms: rabbit anti-calnexin antibody, CANX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90

Purification: Anti-Calnexin was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Calnexin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Calnexin.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-Calnexin antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~67.7 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.2 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.34 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: CANX (CANX Products)

Background: Background: Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin. Anti-Calnexin antibody is ideal for researchers interested in ER Markers, Neuroscience, and Vision research.

Gene ID: 821

NCBI Accession: NP_001019820

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis