Huntingtin antibody (C-Term)

Catalog No. ABIN1607891

Supplier Product No.: 600-401-j24

The Rabbit Polyclonal anti-Huntingtin antibody has been validated for IHC, WB, ELISA and FM. It is suitable to detect Huntingtin in samples from Human, Rat and Mouse.

Quick Overview for Huntingtin antibody (C-Term) (ABIN1607891)

Target: Huntingtin (HTT)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Huntingtin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Product Details anti-Huntingtin Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-j24

Supplier: Rockland

Purpose: Huntington Antibody

Cross-Reactivity (Details): This antibody is specific towards HTT.

Characteristics: Synonyms: rabbit anti-Huntington antibody, rabbit anti-Huntingtin antibody, HD protein, HTT, Huntington Disease Protein, Huntington disease, IT15, LOMARS

Purification: Anti-Huntingtin was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Huntington disease protein.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-Huntingtin antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for Huntingtin

Target: Huntingtin (HTT)

Alternative Name: HTT

Background: Background: Huntington's disease (HD) is an autosomal dominant neurological disorder caused by a polyglutamine (polyQ) repeat expansion in the huntingtin (HTT) protein. The disease is characterized by neurodegeneration and formation of neuronal intracellular inclusions primarily in the striatum and cortex, leading to personality changes, motor impairment, and dementia. The Huntington's disease protein is ~350kD in size and is localized in the brain. Anti-Huntingtin antibodies are ideal for researchers interested in Apoptosis, Autophagy, Cytoskeleton, Neurodegeneration, Neuroscience, and Neuronal Cell Markers research.

Gene ID: 3064

NCBI Accession: NP_002102

UniProt: P42858

Pathways: PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport