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NPM1 antibody (AA 81-294)

This Mouse Monoclonal antibody specifically detects NPM1 in WB, IHC, IF, ELISA, FACS and ICC. It exhibits reactivity toward Human.

Western blot analysis: The HeLa and HepG2 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1000). (NPM1 antibody (AA 81-294))

Immunohistochemistry This image shows paraffin-embedded human palatine tonsil tissue sample stained with anti-NPM antibody(5E3) at 1:100 dilution. (NPM1 antibody (AA 81-294))

IHC (p)

This image shows paraffin-embedded human palatine tonsil tissue sample stained with anti-NPM antibody (5E3) at 1:100 dilution. (NPM1 antibody (AA 81-294))

IHC

ICC/IF analysis of NPM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NPM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). (NPM1 antibody (AA 81-294))

4 images

Catalog No. ABIN165442

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Quick Overview for NPM1 antibody (AA 81-294) (ABIN165442)

Target

See all NPM1 Antibodies

NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivity

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

This NPM1 antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone

5E3

Discover our top product NPM1 Primary Antibody

Binding Specificity
47

32

24

15

15

8

7

5

5

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 81-294

Purpose

Human NPM1 antibody

Purification

Purified

Immunogen

Recombinant human NPM (81-294aa) purified from E. coli

Isotype

IgG2b kappa
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 1-294) antibody
NPM1 Reactivity: Human WB, IF, ELISA, IHC (p) Host: Mouse Monoclonal 3B2 unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody
NPM1 Reactivity: Human WB, IF, FACS, IHC (p), IP Host: Rabbit Polyclonal unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 20-154) antibody
NPM1 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 212-292) antibody
NPM1 Reactivity: Mouse WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (pThr199) antibody
NPM1 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibody
NPM1 Reactivity: Human WB, IHC, IF, ELISA, Coat, StM Host: Mouse Monoclonal NPM1-3286 unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (C-Term) antibody
Verified NPM1 Reactivity: Human, Mouse WB, IHC, IF, ELISA Host: Goat Polyclonal unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibody
NPM1 Reactivity: Human WB, IHC, ELISA, Coat, StM Host: Mouse Monoclonal NPM1-1902 unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibody
NPM1 Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal rNPM1-1901 unconjugated Recombinant Antibody

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 198-226) antibody
NPM1 Reactivity: Human WB, IHC, IF, ELISA, FACS Host: Rabbit Polyclonal unconjugated

Application Notes

Recommended dilution 1:1000(W.B), Recommended dilution 1:100(IHC)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % glycerol

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C,-80 °C

Storage Comment

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Target

NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name

NPM1

Background

Nucleophosmin (NPM, also known as B23, NO38, or numatrin) is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. Nucleophosmin is upregulated in many cancer cells.

NCBI Accession

NP_002511

Pathways

Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly