Manic Fringe antibody (AA 221-321) (AbBy Fluor® 488)

Catalog No. ABIN1695445

This Rabbit Polyclonal antibody specifically detects Manic Fringe in FACS, IF (cc) and IF (p). It exhibits reactivity toward Mouse.

Quick Overview for Manic Fringe antibody (AA 221-321) (AbBy Fluor® 488) (ABIN1695445)

Target: Manic Fringe (MFNG) (MFNG)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Manic Fringe antibody is conjugated to AbBy Fluor® 488

Application: Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Manic Fringe Antibody (AbBy Fluor® 488)

Binding Specificity: AA 221-321

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Sheep,Horse,Chimpanzee

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Manic Fringe/MFNG

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Manic Fringe

Target: Manic Fringe (MFNG) (MFNG)

Alternative Name: Manic Fringe/MFNG

Background:

Synonyms: 3-N-acetylglucosaminyltransferase manic fringe, Beta-1, Beta-1,3-N-acetylglucosaminyltransferase manic fringe, MFNG, MFNG_HUMAN, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase.

Background: Three mammalian fringe family members, Manic, Radical and Lunatic Fringe, have been identified as proteins related to Drosophila fringe, a protein involved in development. Fringe proteins act upstream of the Notch signaling pathway and are involved in boundary determination during segmentation. Each mammalian fringe displays different patterns of expression, though all are expressed in the mouse embryo as well as in many adult tissues. Radical fringe plays a key role in the development of the limb bud. Lunatic fringe is required for normal somite segmentation and patterning and is thought to be a target of the molecular clock. Manic fringe, also involved in somatic development, has been shown to render mouse NIH/3T3 cells tumorigenic in SCID mice.

Gene ID: 4242

Pathways: Notch Signaling