Manic Fringe antibody (Internal Region)

Catalog No. ABIN6258883

The Rabbit Polyclonal anti-Manic Fringe antibody (ABIN6258883) specifically detects Manic Fringe in WB, ICC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Manic Fringe antibody (Internal Region) (ABIN6258883)

Target: Manic Fringe (MFNG) (MFNG)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Manic Fringe antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Manic Fringe Antibody

Binding Specificity: Internal Region

Purpose: MFNG Antibody

Specificity: MFNG Antibody detects endogenous levels of total MFNG.

Predicted Reactivity: Pig,Bovine,Horse,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MFNG, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Manic Fringe

Target: Manic Fringe (MFNG) (MFNG)

Alternative Name: MFNG

Background:

Description: Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules (PubMed:10935626). Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1 (By similarity).

Gene: MFNG

Molecular Weight: 38 kDa

Gene ID: 4242

UniProt: O00587

Pathways: Notch Signaling