FAN1 antibody (AbBy Fluor® 647)

Catalog No. ABIN1698843

The AbBy Fluor® 647-conjugated Rabbit Polyclonal anti-FAN1 antibody (ABIN1698843) specifically detects FAN1 in WB. The antibody is reactive with Human samples.

Quick Overview for FAN1 antibody (AbBy Fluor® 647) (ABIN1698843)

Target: FAN1 (FANCD2/FANCI-Associated Nuclease 1 (FAN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAN1 antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB)

Product Details anti-FAN1 Antibody (AbBy Fluor® 647)

Purpose: FAN1 Polyclonal Antibody, AbBy Fluor-647 Conjugated

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FAN1

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FAN1

Target: FAN1 (FANCD2/FANCI-Associated Nuclease 1 (FAN1))

Alternative Name: Fan1

Background:

Synonyms: KMIN, hFAN1, MTMR15, KIAA1018, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15, FAN1

Background: Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:20671156, PubMed:24981866, PubMed:25430771). Not involved in DNA double-strand breaks resection (PubMed:20603015, PubMed:20603016). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL (PubMed:25430771). Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (PubMed:25430771). Also has endonuclease activity toward 5'-flaps (PubMed:20603015, PubMed:20603016, PubMed:24981866).

Gene ID: 22909

UniProt: Q9Y2M0

Pathways: DNA Damage Repair