SMAD1 antibody (pSer465) (Cy5)

Catalog No. ABIN1704456

Product Details anti-SMAD1 Antibody (Cy5)

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Binding Specificity: pSer465

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD1 antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Ser465

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: Smad1 (SMAD1 Products)

Synonyms: MLP antibody, Xmad1 antibody, Xsmad1 antibody, bsp1 antibody, jv4-1 antibody, jv41 antibody, madh1 antibody, madr1 antibody, smad-1 antibody, MADH1 antibody, fb39h09 antibody, wu:fb39h09 antibody, wu:fb97e04 antibody, 2/23 antibody, CG12399 antibody, Dmel\\CG12399 antibody, E(zen)2 antibody, En(vvl) antibody, MAD antibody, Mat antibody, P-Mad antibody, P-mad antibody, PMad antibody, Smad antibody, Smad1 antibody, apg antibody, c28 antibody, dMAD antibody, dMad antibody, l(2)K00237 antibody, l(2)k00237 antibody, mad antibody, p-Mad antibody, pMAD antibody, pMad antibody, pSmad antibody, BSP-1 antibody, BSP1 antibody, JV4-1 antibody, JV41 antibody, MADR1 antibody, AI528653 antibody, Madh1 antibody, Madr1 antibody, Mlp1 antibody, MusMLP antibody, smad1 antibody, xmad antibody, SMAD family member 1 antibody, Mothers against dpp antibody, SMAD family member 1 L homeolog antibody, smad1 antibody, SMAD1 antibody, Mad antibody, Smad1 antibody, smad1.L antibody

Background:

Synonyms: Smad1 phospho S465, Smad1 phospho Ser465, p-Smad1 Ser465, Mothers against decapentaplegic homolog 1, BSP 1, BSP1, BSP-1, Dwarfin A, DwfA, hSMAD 1, hSMAD1, JV 41, JV4 1, JV4-1, JV41, MAD mothers against decapentaplegic homolog 1, Mad related protein 1, MADH 1, MADH1, Madr 1, MADR1, Mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, Sma and Mad related protein 1, SMAD 1, SMAD-1, SMAD family member 1, SMAD mothers against DPP homolog 1 Drosophila, Spinal muscular atrophy distal with upper limb predominance, TGF beta signaling protein 1, Transforming growth factor beta signaling protein 1, SMAD1_HUMAN, MAD homolog 1, Mothers against DPP homolog 1, Mad-related protein 1, SMAD family member 1, Smad1, Transforming growth factor-beta-signaling protein 1.

Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq].

Gene ID: 4086

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development