MCM7 antibody (AA 1-100) (HRP)

Catalog No. ABIN1711258

This Rabbit Polyclonal antibody specifically detects MCM7 in ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for MCM7 antibody (AA 1-100) (HRP) (ABIN1711258)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM7 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MCM7 Antibody (HRP)

Binding Specificity: AA 1-100

Predicted Reactivity: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CDC47

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7/CDC47

Background:

Synonyms: CDABP0042, CDC 47, CDC47, CDC47 homolog, DNA replication licensing factor MCM7, Homolog of S. cerevisiae Cdc47, MCM 2, MCM 7, MCM2, Mcm7, MCM7 minichromosome maintenance deficient 7, MCM7_HUMAN, Minichromosome Maintainence 7, Minichromosome maintenance complex component 7, Minichromosome maintenance deficient 7, Minichromosome maintenance protein 7, P1.1 MCM3, P1.1-MCM3, P1CDC47, P85MCM, PNAS 146, PNAS146.

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008].

Gene ID: 4176

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA