CSK antibody (pSer364) (HRP)

Catalog No. ABIN1711846

This Rabbit Polyclonal antibody specifically detects CSK in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Rat.

Quick Overview for CSK antibody (pSer364) (HRP) (ABIN1711846)

Target: CSK (C-Src tyrosine Kinase (CSK))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSK antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CSK Antibody (HRP)

Binding Specificity: pSer364

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CSK around the phosphorylation site of Ser364

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Alternative Name: CSK

Background:

Synonyms: CSKphospho S364, p-CSKphospho S364, C SRC, C SRC kinase, C src Tyrosine Kinase, C-SRC kinase, c-src tyrosine kinase, Csk A, CSK, CSK_HUMAN, CYTOPLASMIC TYROSINE KINASE, EC 2.7.10.2, MGC112926, MGC117393, MGC154049, P60 Src, Protein tyrosine kinase CYL, Protein-tyrosine kinase CYL, Proto oncogene tyrosine protein kinase, Tyrosine protein kinase CSK, Tyrosine-protein kinase CSK, zgc:154049.

Background: All members of the Src gene family of tyrosine kinases are characterized by a carboxy terminal domain tyrosine which is highly phosphorylated in the inactive form of the enzyme and phosphorylated to a much lesser extent when the enzyme is active. In the case of Src p60, Y527 is this tyrosine, however, a mutant form of c-Src in which Y527 is replaced by phenylalanine is transforming and displays 5- to 10-fold elevated kinase activity compared to its normal counterpart. Csk has been identified as a Src-related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences amino terminal to the SH3 domain as well as carboxy terminal regulatory sequences. Csk phosphorylates Src on Y527 and also downregulates Lyn, Fyn and Lck by tyrosine phosphorylation of carboxy terminal regulatory sites.

Gene ID: 1445

Pathways: TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events