CSK antibody (AA 330-450)

Catalog No. ABIN94268

Product Details anti-CSK Antibody

Target: CSK (C-Src tyrosine Kinase (CSK))

Binding Specificity: AA 330-450

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CSK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa intracellular non-receptor protein tyrosine kinase.

Cross-Reactivity (Details): Human, Mouse, Other not determined

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.

Clone: CSK-04

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line.
Western blotting: The recommended dilution of the antibody CSK-04 is 1:250-1:300, weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions.
Immunoprecipitation: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Alternative Name: Csk (CSK Products)

Background: C-terminal Src kinase,C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them it lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases, that are localized in lipid rafts, is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.

Gene ID: 1445

UniProt: P41240

Pathways: TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events