CSK antibody (C-Src tyrosine Kinase) (AA 330-450)

Details for Product anti-CSK Antibody No. ABIN94268
Antigen
Epitope
AA 330-450
34
22
16
12
9
9
7
7
5
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
190
116
102
9
8
5
4
2
2
2
1
1
1
Host
Mouse
154
30
9
Clonality (Clone)
Monoclonal ()
Conjugate
This CSK antibody is un-conjugated
10
7
7
5
5
5
5
5
5
5
5
4
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
150
80
32
26
21
18
13
11
10
4
1
1
1
Options
Immunogen Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.
Clone CSK-04
Isotype IgG1
Specificity The antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol.
Cross-Reactivity (Details) Human, Mouse, Other not determined
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Csk (CSK Antibody Abstract)
Background C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.
Pathways TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-C-Src tyrosine Kinase (CSK) (AA 330-450) antibody (ABIN94268) Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk (CSK-04 ; g...