GPR31 antibody (AA 121-220)

Catalog No. ABIN1713499

This Rabbit Polyclonal antibody specifically detects GPR31 in WB, ELISA, IHC (p), ICC, IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for GPR31 antibody (AA 121-220) (ABIN1713499)

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GPR31 Antibody

Binding Specificity: AA 121-220

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human G protein coupled receptor 31

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GPR31

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Alternative Name: GPR31

Background:

Synonyms: HETER, HETER1, 12-HETER, 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor, 12-(S)-HETE receptor, G-protein coupled receptor 31, GPR31

Background: High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.

Gene ID: 2853

UniProt: O00270