STAP2 antibody (AA 151-250)

Catalog No. ABIN1713576

This anti-STAP2 antibody is a Rabbit Polyclonal antibody detecting STAP2 in WB, ELISA, IHC (p), IF (cc), IF (p), ICC and IHC (fro). Suitable for Human.

Quick Overview for STAP2 antibody (AA 151-250) (ABIN1713576)

Target: STAP2 (Signal Transducing Adaptor Family Member 2 (STAP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-STAP2 Antibody

Binding Specificity: AA 151-250

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human STAP2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for STAP2

Target: STAP2 (Signal Transducing Adaptor Family Member 2 (STAP2))

Alternative Name: STAP2

Background:

Synonyms: BKS, Breast tumor kinase substrate, BRK substrate, Signal transducing adaptor protein 2, Signal-transducing adaptor protein 2, Ssignal transducing adaptor family member 2, STAP 2, STAP-2, STAP2, STAP2_HUMAN.

Background: Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues (1,2). Kinases are capable of influencing the oncogenic potential of cell sytems at the level of oncoprotein or tumor suppressor protein phosphorylation states (1,2). STAP-2 is a protein that contains a pleckstrin homology (PH) domain and an SH2 domain, and associates with BRK (3). BRK (breast tumor kinase, Sik) is a 451 amino acid, nonreceptor protein-tyrosine kinase that is overexpressed in breast tumors and metastatic melanoma cell lines (4). Similar to the Src family of intracellular kinses, BRK is comprised of an SH3 domain, an SH2 domain, and a catalytic domain (5). STAP-2 is susceptiple to tyrosine phosphorylation and may be invovled in tyrosine kinase-mediated signaling cascades, whose aberrant function may lead to metastis (3).

Gene ID: 55620

UniProt: Q9UGK3