KRT8 antibody (pSer431)

Catalog No. ABIN1713617

The Rabbit Polyclonal anti-KRT8 antibody has been validated for WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc) and IF (p). It is suitable to detect KRT8 in samples from Human and Mouse.

Quick Overview for KRT8 antibody (pSer431) (ABIN1713617)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-KRT8 Antibody

Binding Specificity: pSer431

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CK8 around the phosphorylation site of Ser431

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background:

Synonyms: Cytokeratin 8 phospho S431, p-Cytokeratin 8 phospho S431, card2, Cardiac autoantigen 2 120kD, CK 8, CK-8, ck8, Cyk 8, cyk8, CYKER, Cytokeratin 8, Cytokeratin endo A, Cytokeratin-8, Cytokeratin8, DreK8, EndoA, k0, CYK8, k2c8, K2C8_HUMAN, k8, Keratin 8, Keratin type ii cytoskeletal 8, Keratin, type II cytoskeletal 8, Keratin-8, Keratin8, KO, Krt 2.8, Krt 8, krt8, KRT-8, MGC118110, MGC174782, MGC53564, MGC85764, sb:cb186, Type-II keratin Kb8.

Background: This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].

Gene ID: 3856