Actin antibody (AA 165-260)

Catalog No. ABIN1713700

This Rabbit Polyclonal antibody specifically detects Actin in WB, ELISA, IHC (p), IF (cc), IF (p), FACS, ICC and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse, Rabbit and Dog and has been mentioned in 12+ publications.

Quick Overview for Actin antibody (AA 165-260) (ABIN1713700)

Target: Actin

Reactivity: Human, Rat, Mouse, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Actin Antibody

Binding Specificity: AA 165-260

Specificity: Alpha, Beta and Gamma Actins

Cross-Reactivity: Dog, Human, Mouse, Rabbit, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human alpha smooth muscle Actin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Actin antibody (ABIN1713700)

Wang, Du, Niu, Fu, Wang, Zhang, Zhao, Sun, Nan: "MiR-130a-3p attenuates activation and induces apoptosis of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis by directly targeting TGFBR1 and TGFBR2." in: Cell death & disease, Vol. 8, Issue 5, pp. e2792, (2018) (PubMed).

Park, Kim: "Cigarette smoke-induced EGFR activation promotes epithelial mesenchymal migration of human retinal pigment epithelial cells through regulation of the FAK-mediated Syk/Src pathway." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 3563-3574, (2018) (PubMed).

Shen, Wu, Wang, Zhao, Zhang, Li, Zhao: "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." in: International immunopharmacology, Vol. 46, pp. 16-22, (2017) (PubMed).

Kim, Ko, Park, Hur, Kim, Yang: "Vandetanib and ADAM inhibitors synergistically attenuate the pathological migration of EBV-infected retinal pigment epithelial cells by regulating the VEGF-mediated MAPK pathway." in: Experimental and therapeutic medicine, Vol. 13, Issue 4, pp. 1415-1425, (2017) (PubMed).

Pang, Bai, Wu, Chen, Zhang, Tang: "Urotensin II Induces ER Stress and EMT and Increase Extracellular Matrix Production in Renal Tubular Epithelial Cell in Early Diabetic Mice." in: Kidney & blood pressure research, Vol. 41, Issue 4, pp. 434-49, (2016) (PubMed).

Gan, Jing, Zhu, Qin, Teng, Guo: "Preventive effects of ¹²⁵I seeds on benign restenosis following esophageal stent implantation in a dog model." in: Molecular medicine reports, Vol. 11, Issue 5, pp. 3382-90, (2016) (PubMed).

Li, Li, Xu, Zhao, Deng, Mo, Li, Qi, Li, Gao: "Total extract of Yupingfeng attenuates bleomycin-induced pulmonary fibrosis in rats." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 22, Issue 1, pp. 111-9, (2015) (PubMed).

Qiao, Wang, Wang, Zhao, Zhang, Han, Peng: "Intermedin is upregulated and attenuates renal fibrosis by inhibition of oxidative stress in rats with unilateral ureteral obstruction." in: Nephrology (Carlton, Vic.), (2015) (PubMed).

Yan, Wu, Li, Meng, Dai, Zheng, Fu: "Cyanidin-3-O-glucoside Induces Apoptosis and Inhibits Migration of Tumor Necrosis Factor-?-Treated Rat Aortic Smooth Muscle Cells." in: Cardiovascular toxicology, (2015) (PubMed).

Xu, Li, Zhao, Qi, Li, Gao, Fei: "Total polysaccharide of Yupingfeng protects against bleomycin-induced pulmonary fibrosis via inhibiting transforming growth factor-?1-mediated type I collagen abnormal deposition in rats." in: The Journal of pharmacy and pharmacology, (2014) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Target Details for Actin

Target: Actin

Background:

Synonyms: Actin, alpha skeletal muscle, ACTA1, Alpha-actin-1, Actin, alpha cardiac muscle 1, ACTC1, Alpha-cardiac actin, Actin, aortic smooth muscle, ACTA2, Alpha-actin-2, Actin, gamma-enteric smooth muscle, ACTG2, Actin, cytoplasmic 2, ACTG1, Actin, cytoplasmic 1, Gamma-actin, ACTB, Beta-actin,

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Gene ID: 70

UniProt: P68032